The effects of chromium VI on behaviour and on gene expression on the nematode Steinernema feltiae.

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Resource or Project Abstract

This research suggests that a mechanism is present within this nematode to allow the production of larger numbers of progeny than normal in a contaminated environment, but behavioural trails such as progeny development times, infectively rates and survival rates are reduced as a result of chromium VI exposures. This was true for chromium VI exposures to both the soil dwelling life stage, and to the within host feeding and development stages. Chromium VI influences a range of gene types within S. feltiae. Here six gene groups have thus far been partially observed, four of which have been shown to have significant similarity to genes previously identified in other organisms. All gene groups with similarities to the sequences elucidated in these experiments have known functions within cellular stress responses. The project findings are significant for further research in the area and show considerable potential for (a) the development of suitable Chromium VI soil bioindicators and (b) the elucidation of Chromium VI stress responses at the genetic level.

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Contact Information for This Resource

Dr. Thomae Kakouli-Duarte
Institute of Technology Carlow
Lecturer/research project leader
Dept. Science & Health, Institute of Technology Carlow, Kilkenny Road, Carlow, Co. Carlow, Ireland
Telephone: 00 353 59 9170554
e-mail: thomae.kakouli@itcarlow.ie

Dr. Stephen Boyle
Institute of Technology Carlow
Former Postdoctoral Research
Dept. Science & Health, Insitute of Technology Carlow, Kilkenny Road, Carlow, Co. Carlow, Ireland
Telephone: 00 353 59 9170554
e-mail: thomae.kakouli@itcarlow.ie

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Offline Print Quality Version    STRIVE_38_Boyle_nematodes_prn.pdf  (3.21 Mb)
Project Report Optimised For Online Viewing    STRIVE_38_Boyle_nematodes_web.pdf  (0.91 Mb)

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Author(s)Kakouli-Duarte, T. Boyle, S.
Title Of WebsiteSecure Archive For Environmental Research Data
Publication InformationThe effects of chromium VI on behaviour and on gene expression on the nematode Steinernema feltiae.
Name of OrganisationEnvironmental Protection Agency Ireland
Electronic Address or URL http://erc.epa.ie/safer/resource?id=6ac0dad0-8b7c-102c-9c91-0a68ec663af0
Unique Identifier6ac0dad0-8b7c-102c-9c91-0a68ec663af0
Date of AccessLast Updated on SAFER: 2017-03-24

An example of this citation in proper usage:

Kakouli-Duarte, T. Boyle, S.   "The effects of chromium VI on behaviour and on gene expression on the nematode Steinernema feltiae.". Associated datasets and digitial information objects connected to this resource are available at: Secure Archive For Environmental Research Data (SAFER) managed by Environmental Protection Agency Ireland http://erc.epa.ie/safer/resource?id=6ac0dad0-8b7c-102c-9c91-0a68ec663af0 (Last Accessed: 2017-03-24)

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Access Information For This Resource

SAFER-Data Display URL http://erc.epa.ie/safer/iso19115/display?isoID=111
Resource KeywordsSteinernema feltiae, ecotoxicology, Progeny number counts, development times, infectivity rates, DNA data
EPA/ERTDI/STRIVE Project Code2005-FS-28
EPA/ERTDI/STRIVE Project ThemeLand-use, Soils, and Transport
Resource Availability: Any User Can Download Files From This Resource
Public-Open
Limitations on the use of this ResourcePart or the totality of the data set will not be accessible without prior permission of the Project Coordinator under Institute of Technology Carlow Policy. In the event that national and international colleagues request clones they will have to undertake responsibility for handling the material with all due care as dictated by EPA regulations. DNA sequence information which will be deposited in publicly accessible DNA databases will be freely available as this type of data does not need to be under usage constraints.
Number of Attached Files (Publicly and Openly Available for Download): 2
Project Start Date Wednesday 1st February 2006 (01-02-2006)
Earliest Recorded Date within any attached datasets or digital objects Friday 1st June 2007 (01-06-2007)
Most Recent Recorded Date within any attached datasets or digital objects Saturday 28th March 2009 (28-03-2009)
Published on SAFERWednesday 6th May 2009 (06-05-2009)
Date of Last EditThursday 28th January 2010 at 12:07:27 (28-01-2010)
Datasets or Files Updated On Thursday 28th January 2010 at 12:07:27 (28-01-2010)

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Geographical and Spatial Information Related To This Resource

Description of Geographical Characteristics of This Project or Dataset
Data was collected from DNA sequences from S. feltiae genes: beta-tubulin and differentially expressed genes in response to chromium VI exposure. This data was generated in IT Carlow laboratories. There is no geographical or spatial reference to this.

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Supplementary Information About This Resource

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Lineage information about this project or dataset
The project arose out of the need for development of viable bioindicators and biomarkers for the detection and study of heavy metal pollution in soils in Ireland. Work leading to such datasets has been well developed internationally, but this project constitutes the first of this type nationally.
Supplementary Information
Reproductive potential, time taken for nematodes to develop and the DNA data of beta-tubulin genes from S. feltiae nematodes growing within chromium VI contaminated host cadavers. Reproductive potential, development times, infectivity rates and survival rates of nematodes after exposure to chromium VI in soils. There is also DNA data of differentially expressed genes as a result of chromium VI exposure (subtracted cDNA libarary). There were no geographical features in this data set. For molecular work DNA sequencers and cDNA libarary generation kits were extensively employed. The effects of chromium VI on the biology of S. feltiae. For counts, infectivity rates etc, collection methods and visualisation methods are standard. Statistical analysis involved techniques such as Shapiro-Wilks test, Kolmogorov-Smirnov tests, Kruskal-Wallis tests, ANOVA tests, bivariate correlations such as Kendall's Tau - b as well as mutiple regression analaysis. For DNA analysis, computers based methodology such as Clustalx, Expasy server and the Sequence Manipulation suite, as well as GenBank and Blast servers.

This work was carried out in two different laboratories in IT Carlow. The molecular work was performed in the molecular biology laboratory. Methodology performed here included RNA and DNA extractions, cDNA library generation as well as subtracted cDNA library generation. PCR was performed in this lab, as was gel imaging, polyacrylamide gel electrophoresis and silver staining. The second laboratory was used for non-molecular experiments and procedures such as nematode cultivation and microcosm trials.
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