Recombinant DNA approaches to enhance the activity of the pathway for the degradation of the toxic pollutant styrene in the bioreactor isolate Pseudomonas putida CA-3: State of Knowledge Report.

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Resource or Project Abstract

Large quantities of styrene are used annually in the petro-chemical and polymer processing industries leading to the release of styrene into the environment and the generation of significant quantities of styrene contaminated waste. This poses a significant threat to the environment and the associated potential toxic effects on human health, including carcinogenesis, are a cause of concern. However, a number of microbial species have the ability to degrade styrene, particularly members of the bacterial genus, Pseudomonas, and this has led to significant interest in using microbial degradation to provide a potential solution to styrene waste remediation. The aim of the current project is to improve the rate of flux through the styrene degradation pathway in Pseudomonas putida CA-3 through recombinant DNA manipulations, thus potentially leading to increased styrene degradation.

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Contact Information for This Resource

Dr. Mark O'Mahony
University College Cork

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Att 1    State_of_Knowledge_-Styrene_degradation-_Mark_O'Mahony.pdf   (0.38 Mb)

Suggested Citation Information

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Author(s)O'Mahony, M.
Title Of WebsiteSecure Archive For Environmental Research Data
Publication InformationRecombinant DNA approaches to enhance the activity of the pathway for the degradation of the toxic pollutant styrene in the bioreactor isolate Pseudomonas putida CA-3: State of Knowledge Report.
Name of OrganisationEnvironmental Protection Agency Ireland
Electronic Address or URL http://erc.epa.ie/safer/resource?id=54074f9a-557a-102d-b891-8d8f2407b579
Unique Identifier54074f9a-557a-102d-b891-8d8f2407b579
Date of AccessLast Updated on SAFER: 2019-09-21

An example of this citation in proper usage:

O'Mahony, M.   "Recombinant DNA approaches to enhance the activity of the pathway for the degradation of the toxic pollutant styrene in the bioreactor isolate Pseudomonas putida CA-3: State of Knowledge Report.". Associated datasets and digitial information objects connected to this resource are available at: Secure Archive For Environmental Research Data (SAFER) managed by Environmental Protection Agency Ireland http://erc.epa.ie/safer/resource?id=54074f9a-557a-102d-b891-8d8f2407b579 (Last Accessed: 2019-09-21)

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Access Information For This Resource

SAFER-Data Display URL http://erc.epa.ie/safer/iso19115/display?isoID=143
Resource KeywordsStyrene, phenylacetic acid, waste remediation, biodegradation, biotransformation, poly-hydroxyalkanoates, bio-plastics, Pseudomonas.
EPA/ERTDI/STRIVE Project Code2007-FS-ET-9-M5
EPA/ERTDI/STRIVE Project ThemeEnvironmental Technologies
Resource Availability: Any User Can Download Files From This Resource
Public-Open
Limitations on the use of this ResourceNone
Number of Attached Files (Publicly and Openly Available for Download): 1
Project Start Date Monday 1st October 2007 (01-10-2007)
Earliest Recorded Date within any attached datasets or digital objects Monday 8th October 2007 (08-10-2007)
Most Recent Recorded Date within any attached datasets or digital objects Friday 18th December 2009 (18-12-2009)
Published on SAFERMonday 18th January 2010 (18-01-2010)
Date of Last EditThursday 21st January 2010 at 15:27:04 (21-01-2010)
Datasets or Files Updated On Monday 18th January 2010 at 13:35:16 (18-01-2010)

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Description of Geographical Characteristics of This Project or Dataset
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Supplementary Information About This Resource

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Lineage information about this project or dataset
A number of styrene degrading Pseudomonas strains, including Pseudomonas putida CA-3, have been well-characterised at both the genetic and physiological level, and a significant body of work has identified the catabolic genes involved in the transformation of styrene to phenylacetic acid, and the subsequent transformation of phenylacetic acid to generate cellular energy, as well as aspects of the genetic regulation and physiological control of the process. Recently, it has also been reported that our P. putida CA-3 strain has the ability to redirect styrene catabolism to the production of a biodegradable polymer, known as poly-hydroxyalkanoate (PHA), under appropriate growth conditions, thus promoting the idea of generating a value-added product from a toxic waste material. This project aims to exploit the considerable knowledge base already generated about bacterial styrene degradation and apply recombinant DNA strategies to potentially improve the styrene degradtion capacity and further charaterise the degradation pathway in P. putida CA-3.
Supplementary Information
None
Links To Other Related Resources
None

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